With rapid developments in novel experimental techniques, limits in the resolution, size and complexity of the biological systems that can be investigated have been constantly pushed forward. The availability of new experimental data poses exciting challenges to the theoretical community for developing novel theoretical and computational models that can be used to better interpret experimental results and glean further mechanistic insights. This is particularly clear in the study of membrane biophysics, which involves complex processes that span multiple length and time scales. In this workshop, as part of our series in Mechanistic analysis of biological systems with novel computational models”, we bring together theoretical & computational biophysicists/chemists and leading experimentalists to discuss important problems in membrane biophysics, especially concerning how to best connect theoretical/computational studies with experiments. The subjects of focus include: membrane mechanics and signaling, antimicrobial/cell-penetration peptides/polymers, diffusion in membranes, membrane fusion and remodeling.