The presence of water in biological systems means that cold temperatures can be fatal for organisms. But many organisms have devised ways to stave off the deleterious effects of freezing temperatures. Recognizing biological motifs that prevent ice formation, scientists have developed artificial methods to keep living organisms viable under cryogenic conditions. Although the scientists exploring ice often point to cryopreservation as an application, few have significant contact with those actively seeking methods for cryopreservation. Likewise, practitioners of cryopreservation may not be aware of ongoing research that explores the molecular interactions of water and ice with materials used for cryopreservation. This workshop aims to convene molecular scientists with cryopreservation practitioners to identify and address grand challenges facing cryopreservation research from fundamental molecular science to applications for preservation of cells, tissue, organs, and organisms.